This discussion on The plot of r/[s] vs r (on the basis of EADIE HOFSTEE plot). The slope and an intercept are?a)b)c)both possibled)None of theseCorrect answer is option 'A'. Can you explain this answer? is done on EduRev Study Group by Chemistry Students. The Questions and Answers of The plot of r/[s] vs r (on the basis of EADIE HOFSTEE plot). The slope and an intercept are?a)b)c)both possibled)None of theseCorrect answer is option 'A'. Can you explain this answer? are solved by group of students and teacher of Chemistry, which is also the largest student community of Chemistry. If the answer is not available please wait for a while and a community member will probably answer this soon. You can study other questions, MCQs, videos and tests for Chemistry on EduRev and even discuss your questions like The plot of r/[s] vs r (on the basis of EADIE HOFSTEE plot). The slope and an intercept are?a)b)c)both possibled)None of theseCorrect answer is option 'A'. Can you explain this answer? over here on EduRev! Apart from being the largest Chemistry community, EduRev has the largest solved Question bank for Chemistry. Show In biochemistry, an Eadie–Hofstee diagram (more usually called an Eadie–Hofstee plot) is a graphical representation of the Michaelis–Menten equation in enzyme kinetics. It has been known by various different names, including Eadie plot, Hofstee plot and Augustinsson plot. Attribution to Woolf is often omitted, because although Haldane and Stern[1] credited Woolf with the underlying equation, it was just one of the three linear transformations of the Michaelis–Menten equation that they initially introduced. However, Haldane indicated latter that Woolf had indeed found the three linear forms: "In 1932, Dr. Kurt Stern published a German translation of my book "Enzymes", with numerous additions to the English text. On pp. 119-120, I described some graphical methods, stating that they were due to my friend Dr. Barnett Woolf. [...] Woolf pointed out that linear graphs are obtained when is plotted against , against , or against , the first plot being most convenient unless inhibition is being studied."[2] Derivation of the equation for the plot[edit]The simplest equation for the rate of an enzyme-catalysed reaction as a function of the substrate concentration is the Michaelis-Menten equation, which can be written as follows: in which is the rate at substrate saturation (when approaches infinity, or limiting rate, and is the value of at half-saturation, i.e. for , known as the Michaelis constant. Eadie[3] and Hofstee[4] independently transformed this into straight-line relationships, as follows: Taking reciprocals of both sides of the equation gives the equation underlying the Lineweaver–Burk plot: ·This can be rearranged to express a different straight-line relationship: which shows that a plot of against is a straight line with intercept on the ordinate, and slope (Hofstee plot). In the Eadie plot the axes are reversed, but the principle is the same. These plots are kinetic versions of the Scatchard plot used in ligand-binding experiments.[5] Attribution to Augustinsson[edit]The plot is occasionally attributed to Augustinsson[6] and referred to the Woolf–Augustinsson–Hofstee plot[7][8][9] or simply the Augustinsson plot.[10] However, although Haldane, Woolf or Eadie are not explicitly cited when Augustinsson introduces the versus equation, both the work of Haldane[11] and of Eadie[3] are cited at other places of his work and are listed in his bibliography.[6]: 169 and 171 Effect of experimental error[edit]Experimental error is usually assumed to affect the rate and not the substrate concentration , so is the dependent variable.[12] As a result, both ordinate and abscissa are subject to experimental error, and so the deviations that occur due to error are not parallel with the ordinate axis but towards or away from the origin. As long as the plot is used for illustrating an analysis rather than for estimating the parameters, that matters very little. Regardless of these considerations various authors[13][14][15] have compared the suitability of the various plots for displaying and analysing data. Use for estimating parameters[edit]Like other straight-line forms of the Michaelis–Menten equation, the Eadie–Hofstee plot was used historically for rapid evaluation of the parameters and , but has been largely superseded by nonlinear regression methods that are significantly more accurate and no longer computationally inaccessible. Making faults in experimental design visible[edit]As the ordinate scale spans the entire range of theoretically possible vales, from 0 to one can see at a glance at an Eadie–Hofstee plot how well the experimental design fills the theoretical design space, and the plot makes it impossible to hide poor design. By contrast, the other well known straight-line plots make it easy to choose scales that imply that the design is better than it is. See also[edit]
Footnotes and references[edit]
What is plotted in the Eadie Hofstee plot?2.3 Eadie-Hofstee Plot
Eadie-Hofstee Plot is also known as Woolf-Eadie-Augustinsson-Hofstee or Eadie- Augustinsson plot. This is a graphical representation of enzyme kinetics in which velocity is plotted as a function of the ratio between velocity and substrate concentration.
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