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BSC2010L Terms in this set (40)Where would the majority of the DNA be located within a eukaryotic cell? nucleus What is DNA isolation? the extraction of DNA from viruses or cells In the DNA isolation process, detergent was used to emulsify cellular and nuclear membranes. In the DNA isolation process, how was the DNA stabilized? The test tube was placed in an ice bath. In the DNA isolation process, the filtrate and reagents are kept in an ice bath because cold temperatures are required to stabilize the DNA. In the DNA isolation process, ________ is used to break down the protein complexes and allow the DNA molecules to easily precipitate. meat tenderizer Ethanol cannot be added to the test tube too quickly because it will break up the DNA precipitate. True DNA can be collected on a glass rod in a technique called spooling. In the DNA isolation process, cells are mixed with sodium chloride (i.e. NaCl) because the sodium (Na+) neutralizes the negative charge of DNA. True Process of isolating DNA Rank the following steps in the correct order that you would use to isolate DNA from strawberries. 3- add ice cold ethanol to test tube 1- transfer strawberry filtrate to test tube 4- wait for DNA precipitate to form 5- spool DNA precipitate onto glass rod 2- dissolve meat tenderizer and add it to test tube Homogenization is a process that suspends and disperses the DNA throughout the filtrate. True In order to prevent the DNA from breaking, the glass rod should be spun in one direction. Ethanol is used in the DNA isolation process because it allows the precipitate to form. Detergent is used in the DNA isolation process because it facilitates homogenization. What is TNF alpha? (Select all that apply) It is a protein that helps the body resist some types of cancer. It is a protein that helps the body fight infectious diseases. Gel electrophoresis can be used to separate molecules by (Select all that apply) length. size. charge. Gel electrophoresis is an important tool used in biotechnology for forensics and paternity test. True In gel electrophoresis, how are the DNA samples added to the wells? A micropipet is used. Why does DNA flow toward the positive side of the gel chamber? DNA has a negative charge and is attracted by the positive side. Ethidium bromide is a dye that is used to stain the gel and allows the DNA to be viewed under UV light. True Why is bromophenol blue added to the individual DNA samples? It allows the observer to view how far the DNA samples travel. The electrophoresis buffer is poured over the agarose gel because it charges the DNA samples so they can travel more readily across the chamber. False In gel electrophoresis, what is the agarose used for? making the gel In the gel electrophoresis lab, you determined the ________ of three different DNA samples. genotype It is not necessary to change pipet tips when you micropipet each new DNA sample. False How does the DNA rate of travel differ for small DNA fragments and large DNA fragments? Small fragments travel farther than large fragments. A high voltage rate will cause the DNA fragments to move slowly across the gel. False A DNA fragment with 100 base pairs is smaller than a DNA fragment with 150 base pairs. True Which is the function of DNA polymerase? It is involved in assembly of the daughter strand using the parental strand as a template. DNA polymerase preserves genetic continuity from parents to offspring. Select each of the following that is a role of DNA polymerase in DNA replication. It synthesizes a daughter strand that is complementary to the template strand. It "proof reads" new daughter strands to be sure the bases are in the correct order. Label the parts of the DNA molecule. Nitrogenous base The new strand of nucleotides formed during DNA replication is called the _____ strand. daughter Each newly formed DNA molecule consists of one parental strand and one daughter strand. A standard gel apparatus for electrophoresis is a rectangle that is open on top. Before pouring the liquid agarose solution into the casting tray, you must first apply tape to the two open ends of the casting tray. Which must be done before pouring liquid gel solution into the gel casting tray? the open ends must be taped DNA size is identified by the number of base pairs (bp). A 100 bp DNA fragment and a 200 bp DNA fragment are separated by gel electrophoresis. Which band should be farther from the wells? DNA size is identified by the number of base pairs (bp). A 100 bp DNA fragment and a 200 bp DNA fragment are separated by gel electrophoresis. Which band should be farther from the wells? the 100bp fragment A sample mixed with bromophenol blue is run using agarose gel at a standard voltage and time. The results yield bands of 200 bp, 50 bp, and the bromophenol blue. Which of the three will migrate the shortest distance? A sample mixed with bromophenol blue is run using agarose gel at a standard voltage and time. The results yield bands of 200 bp, 50 bp, and the bromophenol blue. Which of the three will migrate the shortest distance? bromophenol blue When observing the gel at the end of the run period, the DNA band that is closest to the positive electrode is the When observing the gel at the end of the run period, the DNA band that is closest to the positive electrode is the smallest in size. 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This is an example of 2 answers QUESTION what additional enzymes studied in chapter 3 would need to be mass produced for recombinant DNA technology? 10 answers QUESTION What are the causes of Type 1 and 2 diabetes? 15 answers Why is bromophenol added to the individual DNA samples?Why is bromophenol blue added to the individual DNA samples? It allows the observer to view how far the DNA samples travel. The electrophoresis buffer is poured over the agarose gel because it charges the DNA samples so they can travel more readily across the chamber.
Why is bromophenol blue added?Bromophenol blue is useful as a tracking dye in electrophoresis, an industrial dye, a laboratory acid-base indicator and a biological stain. In gel electrophoresis Bromophenol Blue can be used as a color marker and as as a biological stain. Bromophenol Blue can be used to stain proteins and nucleic acids.
Why does DNA flow toward the other side of the gel chamber?DNA fragments are negatively charged, so they move towards the positive electrode. Because all DNA fragments have the same amount of charge per mass, small fragments move through the gel faster than large ones.
What role do hydrogen bonds play in the DNA molecule quizlet?Hydrogen bonds link complementary nitrogenous bases, thus keeping the two strands of DNA parallel to each other.
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Why is bromophenol blue added to the individual dna samples
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