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BSC2010L
Terms in this set (40)
Where would the majority of the DNA be located within a eukaryotic cell?
nucleus
What is DNA isolation?
the extraction of DNA from viruses or cells
In the DNA isolation process, detergent was used to
emulsify cellular and nuclear membranes.
In the DNA isolation process, how was the DNA stabilized?
The test tube was placed in an ice bath.
In the DNA isolation process, the filtrate and reagents are kept in an ice bath because cold temperatures are required to
stabilize the DNA.
Correct
In the DNA isolation process, ________ is used to break down the protein complexes and allow the DNA molecules to easily precipitate.
meat tenderizer
Ethanol cannot be added to the test tube too quickly because it will break up the DNA precipitate.
True
DNA can be collected on a glass rod in a technique called
spooling.
In the DNA isolation process, cells are mixed with sodium chloride (i.e. NaCl) because the sodium (Na+) neutralizes the negative charge of DNA.
True
Process of isolating DNA
Rank the following steps in the correct order that you would use to isolate DNA from strawberries.
3- add ice cold ethanol to test tube
1- transfer strawberry filtrate to test tube
4- wait for DNA precipitate to form
5- spool DNA precipitate onto glass rod
2- dissolve meat tenderizer and add it to test tube
Homogenization is a process that suspends and disperses the DNA throughout the filtrate.
True
In order to prevent the DNA from breaking, the glass rod should be spun
in one direction.
Ethanol is used in the DNA isolation process because
it allows the precipitate to form.
Detergent is used in the DNA isolation process because
it facilitates homogenization.
What is TNF alpha? (Select all that apply)
It is a protein that helps the body resist some types of cancer.
It is a protein that helps the body fight infectious diseases.
Gel electrophoresis can be used to separate molecules by (Select all that apply)
length.
size.
charge.
Gel electrophoresis is an important tool used in biotechnology for forensics and paternity test.
True
In gel electrophoresis, how are the DNA samples added to the wells?
A micropipet is used.
Why does DNA flow toward the positive side of the gel chamber?
DNA has a negative charge and is attracted by the positive side.
Ethidium bromide is a dye that is used to stain the gel and allows the DNA to be viewed under UV light.
True
Why is bromophenol blue added to the individual DNA samples?
It allows the observer to view how far the DNA samples travel.
The electrophoresis buffer is poured over the agarose gel because it charges the DNA samples so they can travel more readily across the chamber.
False
In gel electrophoresis, what is the agarose used for?
making the gel
In the gel electrophoresis lab, you determined the ________ of three different DNA samples.
genotype
It is not necessary to change pipet tips when you micropipet each new DNA sample.
False
How does the DNA rate of travel differ for small DNA fragments and large DNA fragments?
Small fragments travel farther than large fragments.
A high voltage rate will cause the DNA fragments to move slowly across the gel.
False
A DNA fragment with 100 base pairs is smaller than a DNA fragment with 150 base pairs.
True
Which is the function of DNA polymerase?
It is involved in assembly of the daughter strand using the parental strand as a template.
DNA polymerase
preserves genetic continuity from parents to offspring.
Select each of the following that is a role of DNA polymerase in DNA replication.
It synthesizes a daughter strand that is complementary to the template strand.
It "proof reads" new daughter strands to be sure the bases are in the correct order.
Label the parts of the DNA molecule.
Nitrogenous base
Phosphate
Base pair
Hydrogen bond
Deoxyribose sugar
The new strand of nucleotides formed during DNA replication is called the _____ strand.
daughter
Each newly formed DNA molecule consists of
one parental strand and one daughter strand.
A standard gel apparatus for electrophoresis is
a rectangle that is open on top.
Before pouring the liquid agarose solution into the casting tray, you must first
apply tape to the two open ends of the casting tray.
Which must be done before pouring liquid gel solution into the gel casting tray?
the open ends must be taped
DNA size is identified by the number of base pairs (bp). A 100 bp DNA fragment and a 200 bp DNA fragment are separated by gel electrophoresis. Which band should be farther from the wells?
DNA size is identified by the number of base pairs (bp). A 100 bp DNA fragment and a 200 bp DNA fragment are separated by gel electrophoresis. Which band should be farther from the wells?
the 100bp fragment
A sample mixed with bromophenol blue is run using agarose gel at a standard voltage and time. The results yield bands of 200 bp, 50 bp, and the bromophenol blue. Which of the three will migrate the shortest distance?
A sample mixed with bromophenol blue is run using agarose gel at a standard voltage and time. The results yield bands of 200 bp, 50 bp, and the bromophenol blue. Which of the three will migrate the shortest distance?
bromophenol blue
When observing the gel at the end of the run period, the DNA band that is closest to the positive electrode is the
When observing the gel at the end of the run period, the DNA band that is closest to the positive electrode is the
smallest in size.
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