What is subject variables in research?

The process of examining a research problem in the social and behavioral sciences is often framed around methods of analysis that compare, contrast, correlate, average, or integrate relationships between or among variables. Techniques include associations, sampling, random selection, and blind selection. Designation of the dependent and independent variable involves unpacking the research problem in a way that identifies a general cause and effect and classifying these variables as either independent or dependent.

The variables should be outlined in the introduction of your paper and explained in more detail in the methods section. There are no rules about the structure and style for writing about independent or dependent variables but, as with any academic writing, clarity and being succinct is most important.

After you have described the research problem and its significance in relation to prior research, explain why you have chosen to examine the problem using a method of analysis that investigates the relationships between or among independent and dependent variables. State what it is about the research problem that lends itself to this type of analysis. For example, if you are investigating the relationship between corporate environmental sustainability efforts [the independent variable] and dependent variables associated with measuring employee satisfaction at work using a survey instrument, you would first identify each variable and then provide background information about the variables. What is meant by "environmental sustainability"? Are you looking at a particular company [e.g., General Motors] or are you investigating an industry [e.g., the meat packing industry]? Why is employee satisfaction in the workplace important? How does a company make their employees aware of sustainability efforts and why would a company even care that its employees know about these efforts?

Identify each variable for the reader and define each. In the introduction, this information can be presented in a paragraph or two when you describe how you are going to study the research problem. In the methods section, you build on the literature review of prior studies about the research problem to describe in detail background about each variable, breaking each down for measurement and analysis. For example, what activities do you examine that reflect a company's commitment to environmental sustainability? Levels of employee satisfaction can be measured by a survey that asks about things like volunteerism or a desire to stay at the company for a long time.

The structure and writing style of describing the variables and their application to analyzing the research problem should be stated and unpacked in such a way that the reader obtains a clear understanding of the relationships between the variables and why they are important. This is also important so that the study can be replicated in the future using the same variables but applied in a different way.

Fan, Shihe. "Independent Variable." In Encyclopedia of Research Design. Neil J. Salkind, editor. (Thousand Oaks, CA: SAGE, 2010), pp. 592-594; "What are Dependent and Independent Variables?" Graphic Tutorial; “Case Example for Independent and Dependent Variables.” ORI Curriculum Examples. U.S. Department of Health and Human Services, Office of Research Integrity; Salkind, Neil J. "Dependent Variable." In Encyclopedia of Research Design, Neil J. Salkind, editor. (Thousand Oaks, CA: SAGE, 2010), pp. 348-349; “Independent Variables and Dependent Variables.” Karl L. Wuensch, Department of Psychology, East Carolina University [posted email exchange]; “Variables.” Elements of Research. Dr. Camille Nebeker, San Diego State University.

1 Dept. of Clinical Psychopharmacology and Neurotoxicology, National Institute of Mental Health and Neurosciences, Bengaluru, Karnataka, India.

Find articles by Chittaranjan Andrade

Students without prior research experience may not know how to conceptualize and design a study. This article explains how an understanding of the classification and operationalization of variables is the key to the process. Variables describe aspects of the sample that is under study; they are so called because they vary in value from subject to subject in the sample. Variables may be independent or dependent. Independent variables influence the value of other variables; dependent variables are influenced in value by other variables. A hypothesis states an expected relationship between variables. A significant relationship between an independent and dependent variable does not prove cause and effect; the relationship may partly or wholly be explained by one or more confounding variables. Variables need to be operationalized; that is, defined in a way that permits their accurate measurement. These and other concepts are explained with the help of clinically relevant examples.

Keywords: Independent variable, dependent variable, confounding variable, operationalization of variables, hypothesis

This article explains the following concepts: Independent variables, dependent variables, confounding variables, operationalization of variables, and construction of hypotheses.

In any body of research, the subject of study requires to be described and understood. For example, if we wish to study predictors of response to antidepressant drugs (ADs) in patients with major depressive disorder (MDD), we might select patient age, sex, age at onset of MDD, number of previous episodes of depression, duration of current depressive episode, presence of psychotic symptoms, past history of response to ADs, and other patient and illness characteristics as potential predictors. These characteristics or descriptors are called variables. Whether or not the patient responds to AD treatment is also a variable. A solid understanding of variables is the cornerstone in the conceptualization and preparation of a research protocol, and in the framing of study hypotheses. This subject is presented in two parts. This article, Part 1, explains what independent and dependent variables are, how an understanding of these is important in framing hypotheses, and what operationalization of a variable entails.

Variables are defined as characteristics of the sample that are examined, measured, described, and interpreted. Variables are so called because they vary in value from subject to subject in the study. As an example, if we wish to examine the relationship between age and height in a sample of children, age and height are the variables of interest; their values vary from child to child. In the earlier example, patients vary in age, sex, duration of current depressive episode, and response to ADs. Variables are classified as dependent and independent variables and are usually analyzed as categorical or continuous variables.

Independent variables are defined as those the values of which influence other variables. For example, age, sex, current smoking, LDL cholesterol level, and blood pressure are independent variables because their values (e.g., greater age, positive for current smoking, and higher LDL cholesterol level) influence the risk of myocardial infarction. Dependent variables are defined as those the values of which are influenced by other variables. For example, the risk of myocardial infarction is a dependent variable the value of which is influenced by variables such as age, sex, current smoking, LDL cholesterol level, and blood pressure. The risk is higher in older persons, in men, in current smokers, and so on.

There may be a cause–effect relationship between independent and dependent variables. For example, consider a clinical trial with treatment (iron supplement vs placebo) as the independent variable and hemoglobin level as the dependent variable. In children with anemia, an iron supplement will raise the hemoglobin level to a greater extent than will placebo; this is a cause–effect relationship because iron is necessary for the synthesis of hemoglobin. However, consider the variables teeth and weight. An alien from outer space who has no knowledge of human physiology may study human children below the age of 5 years and find that, as the number of teeth increases, weight increases. Should the alien conclude that there is a cause–effect relationship here, and that growing teeth causes weight gain? No, because a third variable, age, is a confounding variable1–3 that is responsible for both increase in the number of teeth and increase in weight. In general, therefore, it is more proper to state that independent variables are associated with variations in the values of the dependent variables rather than state that independent variables cause variations in the values of the dependent variables. For causality to be asserted, other criteria must be fulfilled; this is out of the scope of the present article, and interested readers may refer to Schunemann et al.4

As a side note, here, whether a particular variable is independent or dependent will depend on the question that is being asked. For example, in a study of factors influencing patient satisfaction with outpatient department (OPD) services, patient satisfaction is the dependent variable. But, in a study of factors influencing OPD attendance at a hospital, OPD attendance is the dependent variable, and patient satisfaction is merely one of many possible independent variables that can influence OPD attendance.

Students must have a clear idea about what they want to study in order to conceptualize and frame a research protocol. The first matters that they need to address are “What are my research questions?” and “What are my hypotheses?” Both questions can be answered only after choosing the dependent variables and then the independent variables for study.

In the case of a student who is interested in studying predictors of AD outcomes in patients with MDD, treatment response is the dependent variable and patient and clinical characteristics are possible independent variables. So, the selection of dependent and independent variables helps defines the objectives of the study:

1. To determine whether sociodemographic variables, such as age and sex, predict the outcome of an episode of depression in MDD patients who are treated with an AD.

2. To determine whether clinical variables, such as age at onset of depression, number of previous depressive episodes, duration of current depressive episode, and the presence of soft neurological signs, predict the outcome of an episode of depression in MDD patients who are treated with an AD.

Note that in a formal research protocol, the student will need to state all the independent variables and not merely list examples. The student may also choose to include additional independent variables, such as baseline biochemical, psychophysiological, and neuroradiological measures.

A hypothesis is a clear statement of what the researcher expects to find in the study. As an example, a researcher may hypothesize that longer duration of current depression is associated with poorer response to ADs. In this hypothesis, the duration of the current episode of depression is the independent variable and treatment response is the dependent variable. It should be obvious, now, that a hypothesis can also be defined as the statement of an expected relationship between an independent and a dependent variable. Or, expressed visually, (independent variable) (arrow) (dependent variable) = hypothesis.

It would be a waste of time and energy to do a study to examine only one question: whether duration of current depression predicts treatment response. So, it is usual for research protocols to include many independent variables and many dependent variables in the generation of many hypotheses, as shown in Table 1. Pairing each variable in the “independent variable” column with each variable in the “dependent variable” column would result in the generation of these hypotheses. Table 2 shows how this is done for age. Sets of hypotheses can likewise be constructed for the remaining independent and dependent variables in Table 1. Importantly, the student must select one of these hypotheses as the primary hypothesis; the remaining hypotheses, no matter how many they are, would be secondary hypotheses. It is necessary to have only one hypothesis as the primary hypothesis in order to calculate the sample size necessary for an adequately powered study and to reduce the risk of false positive findings in the analysis.5 In rare situations, two hypotheses may be considered equally important and may be stated as coprimary hypotheses.

Independent Variables and Dependent Variables in a Study on Sociodemographic and Clinical Prediction of Response of Major Depressive Disorder to Antidepressant Drug Treatment

Combinations of Age with Dependent Variables in the Generation of Hypotheses

In Table 1, suicidality is listed as an independent variable and severity of depression, as a dependent variable. These variables need to be operationalized; that is, stated in a way that explains how they will be measured. Table 3 presents three ways in which suicidality can be measured and four ways in which (reduction in) the severity of depression can be measured. Now, each way of measurement in the “independent variable” column can be paired with a way of measurement in the “dependent variable” column, making a total of 12 possible hypotheses. In like manner, the many variables listed in Table 1 can each be operationalized in several different ways, resulting in the generation of a very large number of hypotheses. As already stated, the student must select only one hypothesis as the primary hypothesis.

Possible Ways of Operationalization of Suicidality and Depression

Much thought should be given to the operationalization of variables because variables that are carelessly operationalized will be poorly measured; the data collected will then be of poor quality, and the study will yield unreliable results. For example, socioeconomic status may be operationalized as lower, middle, or upper class, depending on the patient’s monthly income, on the total monthly income of the family, or using a validated socioeconomic status assessment scale that takes into consideration income, education, occupation, and place of residence. The student must choose the method that would best suit the needs of the study, and the method that has the greatest scientific acceptability. However, it is also permissible to operationalize the same variable in many different ways and to include all these different operationalizations in the study, as shown in Table 3. This is because conceptualizing variables in different ways can help understand the subject of the study in different ways.

Operationalization of variables requires a consideration of the reliability and validity of the method of operationalization; discussions on reliability and validity are out of the scope of this article. Operationalization of variables also requires specification of the scale of measurement: nominal, ordinal, interval, or ratio; this is also out of the scope of the present article. Finally, operationalization of variables can also specify details of the measurement procedure. As an example, in a study on the use of metformin to reduce olanzapine-associated weight gain, we may state that we will obtain the weight of the patient but fail to explain how we will do it. Better would be to state that the same weighing scale will be used. Still better would be to state that we will use a weighing instrument that works on the principle of moving weights on a levered arm, and that the same instrument will be used for all patients. And best would be to add that we will weigh patients, dressed in standard hospital gowns, after they have voided their bladder but before they have eaten breakfast. When the way in which a variable will be measured is defined, measurement of that variable becomes more objective and uniform

The next article, Part 2, will address what categorical and continuous variables are, why continuous variables should not be converted into categorical variables and when this rule can be broken, and what confounding variables are.

Declaration of Conflicting Interests: The author declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding: The author received no financial support for the research, authorship, and/or publication of this article.

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